American shad Alosa sapidissima Microsatellite Primers

shad.jpg (297646 bytes)

References:
Waters, J., J. Epifanio, T. Gunter and B. Brown. 2000. Subtle population differentiation in Alosa sapidissima revealed by microsatellite DNA and mtDNA analysis. J. Fish Biol. in press.

Brown, B., T. Gunter, J. Waters, J. Epifanio. 2000. Evaluating genetic diversity associated with propagation-assisted restoration of American shad. Conser. Biol. in press.

 

Details of the six DNA microsatellites of American shad, Alosa sapidissima

Annealing

Allele

Number of

GenBank

temperature

size-range

alleles

accession
Locus

Repeat motifs

Primer sequence (5'-3')

(°C)

(bp)

observed

number
Asa-2

(TTC)13

 CATTACTCCAAGTTGCTTTTATTT

48

73-133

19

AF039657
GAGATGACAGAAGAATTGAAGAGA
Asa-4

(ACC)3(AAC)12(AGC)6

 GAAGACAATACAGTAATAAACC

50

124-172

17

AF039658
GCGGGAGGCCAGACATA
Asa-6

(AAC)12

 ACCTTCTGTTCTGTTTCACCTG

47

83-110

10

AF039659
TTCACTGTAATGCAATGTAATGTT
Asa-8

(TTTG)8

 TCCATTCCATTACGTAGAGCACT

50

108-168

15

AF039660
CCGGCAGGGCACAGAAC
Asa-9

(TTTC)7

 GGGAATAAGGGATGTAGCCAAGAT

50

156-266

20

AF039661
AGGAGAAGGAAAGGGGAGTGAGAG
Asa-16

(GTT)3(CCT)(GTT)12

 CCGTCGCCGATGTAGTTGCAGTGG

54

116-131

6

AF049462
ACGGGAGAAAAGAGTATGTGTCCTTGAG

 

2,4,6.gif (186168 bytes)

Asa-8   Heteroduplexes are exemplified by arrows.

asa-8.gif (26090 bytes)

9,16.gif (130772 bytes)

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Revised: 12/10/98